VAV3 Antibody (OAEB01225)

Catalog No: OAEB01225 (Formerly GWB-AC9D03)

Size:100UG

OAEB01225 (1ug/ml) staining of human bone marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

(7.5ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Reactivity Info: Tested: Human; Expected: Human, Dog
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: VAV3
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%
• Peptide Sequence: C-SGEQGTLKLPEK
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:128000.
  Western Blot: Approx 80kDa band observed in human bone marrow lysates (calculated MW of 97.8kDa according to NP_006104). Recommended concentration: 0.3-1ug/ml.
• Reference: * Salhia B, Tran NL, Chan A, Wolf A, Nakada M, Rutka F, Ennis M, McDonough WS, Berens ME, Symons M, Rutka JT.; The guanine nucleotide exchange factors trio, Ect2, and Vav3 mediate the invasive behavior of glioblastoma.; Am J Pathol. 2008 Dec;173(6):1828-38.; Pubmed 19008376
  * Movilla N, Bustelo XR.; Biological and regulatory properties of Vav-3, a new member of the Vav family of oncoproteins.; Mol Cell Biol. 1999 Nov;19(11):7870-85.; Pubmed 10523675

Target Info

• Gene Symbol: VAV3
• Gene Full Name: vav guanine nucleotide exchange factor 3
• Alias Symbols: guanine nucleotide exchange factor VAV3;vav 3 guanine nucleotide exchange factor;vav 3 oncogene;VAV-3.
• NCBI Gene Id: 10451
• Protein Name: Guanine nucleotide exchange factor VAV3
• Uniprot ID: Q9UKW4
• Protein Accession #: NP_006104.4
• Molecular Weight: 97.8; 32.6