VAV1 Antibody (OAGA08422)

Catalog No: OAGA08422

Size:100 ul

VAV1 antibody [GT557] detects VAV1 protein by Western blot analysis.
A. 30 ug Jurkat whole cell lysate/extract
B. 30 ug NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
VAV1 antibody [GT557] dilution: 1:1000

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Monoclonal
• Clone: GT557
• Isotype: IgG2b
• Host: Mouse
• Application: WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human VAV1. The exact sequence is proprietary.
• Purification: Affinity purified by Protein G.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: PBS

Target Info

• Gene Symbol: VAV1
• Gene Full Name: vav guanine nucleotide exchange factor 1
• Alias Symbols: VAV
• NCBI Gene Id: 7409
• Protein Name: proto-oncogene vav
• Description of Target: This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Uniprot ID: P15498
• Protein Accession #: NP_001245136.1
• Nucleotide Accession #: NM_001258207.1