VAV1 Antibody (OAAB22246)

Catalog No: OAAB22246

Size:200ul

VAV1 Antibody (OAAB22246) at 1:4,000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution. Predicted band size: 98 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Clone: 1582CT802.383.58
• Isotype: IgG1, kappa
• Host: Mouse
• Application: WB
• Additional Information: Function: Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This VAV1 antibody is generated from a mouse immunized with a recombinant protein of human VAV1.
• Purification: Protein G column purification, followed by dialysis against PBS
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Concentration: 500 ug/mL
• Preservative: 0.09% Sodium Azide
• Formulation: Purified monoclonal antibody, supplied in PBS with 0.09% sodium azide.
• Application Info: WB: 1:4000
• Reference: Coppola J.,et al.Cell Growth Differ. 2:95-105(1991).
  Denkinger D.J.,et al.Biochim. Biophys. Acta 1491:253-262(2000).
  Grimwood J.,et al.Nature 428:529-535(2004).
  Katzav S.,et al.Mol. Cell. Biol. 11:1912-1920(1991).
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  

Target Info

• Gene Symbol: VAV1
• Gene Full Name: vav guanine nucleotide exchange factor 1
• Alias Symbols: VAV
• NCBI Gene Id: 7409
• Description of Target: This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Uniprot ID: P15498
• Protein Accession #: NP_001245136.1
• Nucleotide Accession #: NM_001258207.1
• Molecular Weight: 98 kDa