VASP ELISA Kit (Human) (OKCD08196)

Catalog No: OKCD08196

Size:96WELLS

VASP ELISA Kit (Human) (OKCD08196) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vasodilator Stimulated Phosphoprotein (VASP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vasodilator Stimulated Phosphoprotein (VASP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vasodilator Stimulated Phosphoprotein (VASP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Vasodilator Stimulated Phosphoprotein (VASP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vasodilator Stimulated Phosphoprotein (VASP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vasodilator Stimulated Phosphoprotein (VASP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • VASP Microplate: 96 Wells (12 x 8 Well strips)
  • VASP Lyophilized Standard: 2
  • 100X VASP HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.056ng/mL

Target Info

• Gene Symbol: VASP
• Gene Full Name: vasodilator stimulated phosphoprotein
• Alias Symbols: vasodilator-stimulated phosphoprotein.
• NCBI Gene Id: 7408
• Protein Name: Vasodilator-stimulated phosphoprotein
• Description of Target: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
• Uniprot ID: P50552
• Protein Accession #: NP_003361.1
• Nucleotide Accession #: NM_003370.3