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Catalog No: OKEH03950
Size:96T
Price: $625.00
SKU
OKEH03950
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology VASP ELISA Kit (Bovine) (OKEH03950) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human VASP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated VASP and incubated. The VASP found in the sample or standards competes with the biotinylated VASP for limited binding sites on the immobilized anti-human VASP antibody. Excess unbound biotinylated VASP and sample or standard VASP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated VASP captured in the well and inversely proportional to the amount of VASP which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/ml
ELISA Kit Component
ComponentAmount
Anti-VASP Microplate96 Wells (12 x 8 Well strips)
VASP Lyophilized Standard2
Sample Diluent1 x 20 mL
100X VASP-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.091 ng/mL
SpecificityNatural and recombinant Bovine Vasodilator-stimulated phosphoprotein
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolVASP
Gene Full Namevasodilator stimulated phosphoprotein
Alias Symbolsvasodilator-stimulated phosphoprotein.
NCBI Gene Id514902
Protein NameQ2TA49
Description of TargetVasodilator-stimulated phosphoprotein
Uniprot IDhttps://www.uniprot.org/uniprot/Q2TA49
Protein Accession #NP_001033199.1
Nucleotide Accession #NM_001038110.2
Protein Size (# AA)383
Molecular Weight383 kDa
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