Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH01201
Size:96T
Price: $525.00
SKU
OKEH01201
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology VASN ELISA Kit (Mouse) (OKEH01201) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VASN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for VASN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VASN captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.0% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20)
ELISA Kit Component
ComponentAmount
VASN Microplate96 Wells (12 x 8 Well strips)
VASN Lyophilized Standard2
100X Biotinylated VASN Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Mouse Vasorin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolVasn
Gene Full Namevasorin
Alias Symbols2610528G05Rik;A;Atia;protein slit-like 2;Sl;Slitl2;Slit-like 2;vasorin.
NCBI Gene Id246154
Protein NameQ9CZT5
Description of TargetVasorin
Uniprot IDhttps://www.uniprot.org/uniprot/Q9CZT5
Protein Accession #NP_647468.2
Nucleotide Accession #NM_139307.3
Protein Size (# AA)673
Molecular Weight72 kDa
Write Your Own Review
You're reviewing:VASN ELISA Kit (Mouse) (OKEH01201)
Your Rating
We found other products you might like!