VAPA Antibody (OAGA00925)

Catalog No: OAGA00925

Size:100 ul

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
OAGA00925 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
VAPA antibody
OAGA00925 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded breast ca, using VAPA(OAGA00925) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded lung AdCA, using VAPA(OAGA00925) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, NIH-3T3
  Cellular Localization: Cell membrane; Single-pass type IV membrane protein, Endomembrane system; Single-pass type IV membrane protein
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 29 and 122 of VAPA (Uniprot ID#Q9P0L0)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: VAPA
• Gene Full Name: Vesicle-associated membrane protein-associated protein A
• Alias Symbols: VAP-A, VAP33, VAMP-A, VAP-33, hVAP-33
• NCBI Gene Id: 9218
• Protein Name: Vesicle-associated membrane protein-associated protein A
• Uniprot ID: Q9P0L0
• Protein Accession #: NP_003565.4
• Molecular Weight: 28 kDa