- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for anti-VAMP2 (OAED00235) antibody |
---|
Predicted Species Reactivity | Human, Mouse, Rat, Bovine, Hamster, Pig, Xenopus |
---|---|
Product Format | Liquid. In PBS, pH 7.2, containing 0.1mM PMSF and 50% glycerol. |
Clonality | Monoclonal |
Clone | SP10 |
Isotype | IgM |
Host | Mouse |
Application | IHC, WB |
Reconstitution and Storage | Long Term Storage : -20C . Avoid freeze-thaw cycles. |
Immunogen | Synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates using anti-human Synaptophysin monoclonal antibodies. |
Purification | Ammonium sulfate precipitation purified. |
Specificity | Recognizes human, mouse, rat, bovine, hamster, pig and Xenopus VAMP2. Detects a band of ~18kDa by Western blot. |
Application Info | Immunohistochemistry (1:50, human neuroblastoma tissue) Western Blot (1:4000, colorimetric) Optimal conditions must be determined individually for each application. |
Reference | Aquaporin 1 regulates GTP-induced rapid gating of water in secretory vesicles: B.P. Jena, et al. ; PNAS 99, 4720 (2002), Application(s): WB using rat samples, Abstract; The 26-mer peptide released from SNAP-25 cleavage by botulinum neurotoxin E inhibits vesicle docking: M. Montal, et al. ; FEBS Lett. 435, 84 (1998), Application(s): WB using rat samples, Abstract; |
Gene Symbol | VAMP2 |
---|---|
Gene Full Name | Vesicle-associated membrane protein 2 |
Alias Symbols | SYB2, VAMP-2, NEDHAHM |
NCBI Gene Id | 6844 |
Protein Name | Vesicle-associated membrane protein 2 |
Description of Target | Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicles. Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system. VAMP2 is found in neurons associated with autonomic, sensory and integrative functions, and in non-neuronal Langerhans islets and glomerular cells. VAMP is a component of a 20S complex implicated in vesicle docking and fusion, the formation of which involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP, and NSF. |
Uniprot ID | P63027, P23763 (VAMP1) |
Protein Accession # | NP_055047.2 |
Nucleotide Accession # | NM_014232.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "VAMP2 Antibody (OAED00235)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Bovine, Hamster, Pig, Xenopus".
-
How long will it take to receive "VAMP2 Antibody (OAED00235)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 week".
-
What buffer format is "VAMP2 Antibody (OAED00235)" provided in?
This item is provided in "Liquid. In PBS, pH 7.2, containing 0.1mM PMSF and 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "VAMP2 Antibody (OAED00235)"?
This target may also be called "SYB2, VAMP-2, NEDHAHM" in publications.
-
What is the shipping cost for "VAMP2 Antibody (OAED00235)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "VAMP2 Antibody (OAED00235)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "VAMP2 Antibody (OAED00235)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "VAMP2 Antibody (OAED00235)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "VAMP2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "VAMP2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "VAMP2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "VAMP2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "VAMP2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "VAMP2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.