Size:96 Wells
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Valine ELISA Kit (OKEH02611)

Catalog#: OKEH02611
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species Reactivity All
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology Valine ELISA Kit (OKEH02611) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Valine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Valine and incubated. The Valine found in the sample or standards competes with the biotinylated Valine for limited binding sites on the immobilized anti-Valine antibody. Excess unbound biotinylated Valine and sample or standard Valine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Valine captured in the well and inversely proportional to the amount of Valine which was contained in the sample or standard.
Kit Range 1.56 - 100nmol/mL
Kit Reproducibility Mean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 7.1% (n = 20)
Kit Component
Anti-Valine Microplate96 Wells (12 x 8 Well strips)
Valine Lyophilized Standard2 x 100 nmol
Sample Diluent1 x 20 mL
100X Valine-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional Information Target Abbr: VAL
Target Name: Valine
:: Pubchem: 6287
:: Chemical formula: C5H11NO2
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity 0.34nmol/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant General Valine
Assay Info Assay Methodology: Quantitative Competitive ELISA
Alias Symbols VAL, Valine, VAL
Protein Name Valine
Description of Target Valine is an aliphatic and extremely hydrophobic essential amino acid in humans related to leucine, Valine is found in many proteins, mostly in the interior of globular proteins helping to determine three-dimensional structure. A glycogenic amino acid, valine maintains mental vigor, muscle coordination, and emotional calm. Valine is obtained from soy, cheese, fish, meats and vegetables. Valine supplements are used for muscle growth, tissue repair, and energy.
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