Catalog No: OPCA03404
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OPCA03404
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Datasheets/ManualsPrintable datasheet for UVSX Recombinant Protein (Enterobacteria phage T5) (OPCA03404)
Product Info
Predicted Species ReactivityBacteriophage T4
Product FormatLiquid or Lyophilized powder
HostEnterobacteria phage T5
Additional InformationRelevance: Important in genetic recombination, DNA repair, and replication. Possesses pairing and strand-transfer activity. Interacts with dda and gene 32 proteins.
Reconstitution and StorageWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Formulation20 mM Tris-HCl based buffer, pH 8.0
PurityGreater than 90% as determined by SDS-PAGE.
Protein SequenceMSDLKSRLIKASTSKLTAELTASKFFNEKDVVRTKIPMMNIALSGEITGGMQSGLLILAGPSKSFKSNFGLTMVSSYMRQYPDAVCLFYDSEFGITPAYLRSMGVDPERVIHTPVQSLEQLRIDMVNQLDAIERGEKVVVFIDSLGNLASKKETEDALNEKVVSDMTRAKTMKSLFRIVTPYFSTKNIPCIAINHTYETQEMFSKTVMGGGTGPMYSADTVFIIGKRQIKDGSDLQGYQFVLNVEKSRTVKEKSKFFIDVKFDGGIDPYSGLLDMALELGFVVKPKNGWYAREFLDEETGEMIREEKSWRAKDTNCTTFWGPLFKHQPFRDAIKRAYQLGAIDSNEIVEAEVDELINSKVEKFKSPESKSKSAADLETDLEQLSDMEEFNE
Storage BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
SourceE. Coli
Protein Range1-391 aa
TagN-terminal 6xHis-SUMO-tagged
ReferenceSequence of the T4 recombination gene, uvsX, and its comparison with that of the recA gene of Escherichia coli.Fujisawa H., Yonesaki T., Minagawa T.Nucleic Acids Res. 13:7473-7481(1985)
Gene SymbolUVSX
Alias SymbolsUVSX,Recombination and repair protein
Uniprot IDP04529
Protein Size (# AA)1-391 aa
Molecular Weight60 kDa
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