UVRAG Antibody (OACA09707)

Catalog No: OACA09707

Immunofluorescent analysis of HepG2 cells at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human liver cancer at dilution of 1:100

Immunohistochemistry of paraffin-embedded human lung cancer at dilution of 1:100

Western blot
All lanes : UVRAG antibody at 2 ug/ml
Lane 1 : SH-SY5Y whole cell lysate
Lane 2 : 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/1,0000 dilution
Predicted band size: 79,36 kDa
Observed band size: 78 kDa

Western blot
All lanes : UVRAG antibody at 2 ug/ml
Lane 1 : SH-SY5Y whole cell lysate
Lane 2 : 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/1,0000 dilution
Predicted band size: 79,36 kDa
Observed band size: 78 kDa

Immunohistochemistry of paraffin-embedded human liver cancer at dilution of 1:100

Immunohistochemistry of paraffin-embedded human lung cancer at dilution of 1:100

Immunofluorescent analysis of HepG2 cells at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human UV radiation resistance-associated gene protein (375-425AA)
• Purification: Protein G Purified
• Preservative: 0.03% Proclin 300
• Formulation: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%

Target Info

• Gene Symbol: UVRAG
• Gene Full Name: UV radiation resistance associated
• Alias Symbols: beclin 1 binding protein;DHTX;disrupted in heterotaxy;p63;UV radiation resistance-associated gene protein;VPS38.
• NCBI Gene Id: 7405
• Protein Name: UV radiation resistance-associated gene protein
• Description of Target: Versatile protein that is involved in regulation of different cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed:24056303). During autophagy acts as regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3. Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2 (PubMed:16799551, PubMed:20643123, PubMed:24056303). Involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depending on its association with a class C Vps complex (possibly the HOPS complex); the association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events (PubMed:18552835). Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion (PubMed:24550300). In case of negative-strand RNA virus infection is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8-specific fusogenic SNARE complex assembly (PubMed:24550300).
• Uniprot ID: Q9P2Y5
• Protein Accession #: NP_003360
• Nucleotide Accession #: NM_003369