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Catalog No: OKCD08864
Size:96WELLS
Price: $800.00
SKU
OKCD08864
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Datasheets/ManualsPrintable datasheet for UTRN ELISA Kit (Human) (OKCD08864)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Utrophin (UTRN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Utrophin (UTRN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Utrophin (UTRN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Utrophin (UTRN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Utrophin (UTRN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Utrophin (UTRN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
UTRN Microplate96 Wells (12 x 8 Well strips)
UTRN Lyophilized Standard2
100X UTRN HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.067ng/mL
Gene SymbolUTRN
Gene Full Nameutrophin
Alias SymbolsDMDL;DRP;DRP1;dystrophin-related protein 1;utrophin.
NCBI Gene Id7402
Protein NameUtrophin
Description of TargetMay play a role in anchoring the cytoskeleton to the plasma membrane.
Uniprot IDP46939
Protein Accession #NP_009055.2
Nucleotide Accession #NM_007124.2
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