USP8 ELISA Kit (Human) (OKCD00957)

Catalog No: OKCD00957 (Formerly GWB-KBBLT1)

Size:96WELLS

Human USP8 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Specific Peptidase 8 (USP8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Specific Peptidase 8 (USP8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Specific Peptidase 8 (USP8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ubiquitin Specific Peptidase 8 (USP8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Specific Peptidase 8 (USP8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Specific Peptidase 8 (USP8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-USP8 Microplate: 96 Wells (12 x 8 Well Strips)
  • USP8 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated USP8 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell Lysates and other biological fluids.
• Sensitivity: < 0.101 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ubiquitin Specific Peptidase 8 (USP8). No significant cross-reactivity or interference between Ubiquitin Specific Peptidase 8 (USP8) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Description:
  View product citations for USP8 ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: USP8
• Gene Full Name: ubiquitin specific peptidase 8
• Alias Symbols: deubiquitinating enzyme 8;HumORF8;PITA4;SPG59;ubiquitin carboxyl-terminal hydrolase 8;ubiquitin isopeptidase Y;Ubiquitin thioesterase 8;ubiquitin thiolesterase 8;ubiquitin-specific-processing protease 8;UBPY.
• NCBI Gene Id: 9101
• Protein Name: Ubiquitin carboxyl-terminal hydrolase 8
• Description of Target: Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1.
• Uniprot ID: P40818
• Protein Accession #: NP_001122082
• Nucleotide Accession #: NM_001128610.2
• Protein Size (# AA): 1118