USP28 Antibody - N-terminal region (OAAB15770)

Catalog No: OAAB15770

Size:400ul

The anti-USP28 Pab (Cat. # OAAB15770) is used in Western blot to detect USP28 in Jurkat cell lysate.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
• Additional Information: Precautions: USP28 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C) In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus
  Cellular Location: Nucleus, nucleoplasm.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This USP28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-141 amino acids from the N-terminal region of human USP28.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000

Target Info

• Gene Symbol: USP28
• Alias Symbols: USP28, KIAA1515, Ubiquitin carboxyl-terminal hydrolase 28, Deubiquitinating enzyme 28, Ubiquitin thioesterase 28, Ubiquitin-specific-processing protease 28
• NCBI Gene Id: 57646
• Protein Name: Ubiquitin carboxyl-terminal hydrolase 28
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q96RU2
• Protein Accession #: NP_065937.1
• Molecular Weight: 122491 Da