USP25 Antibody - C-terminal region (OAAB15768)

Catalog No: OAAB15768

Size:400ul

The anti-USP25 C-term Pab (Cat. # OAAB15768) is used in Western blot to detect USP25 in rat testis tissue lysate (lane 1) and USP25-transfected HeLa cell lysates (lane 2). Transfection data is kindly provided by Dr. B. Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Rat
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: USP25 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains
  Cellular Location: Cytoplasm.
  Tissue Location: Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This USP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1037-1068 amino acids from the C-terminal region of human USP25.
• Predicted Homology Based on Immunogen Sequence: Human; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: USP25
• Alias Symbols: USP21
• NCBI Gene Id: 29761
• Protein Name: Ubiquitin carboxyl-terminal hydrolase 25
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9UHP3
• Protein Accession #: NP_037528.3
• Molecular Weight: 122218 Da