Usp2 Recombinant Protein (Rat) (OPED00161)

Catalog No: OPED00161

Size:100UG

Example of assay result. DUB assay: 50 nM USP2 catalytic domain (BML-UW9850); 500nM Ubiquitin-AMC; 50mM HEPES, pH 7.8, 0.5mM EDTA, 1mM DTT

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Product Format: Liquid. In 10mM MES, 0.5mM DTT, 50mM TRIS-HCl, pH 7.5, containing 20% glycerol.
• Host: E. Coli
• Additional Information: Ubiquitin-specific proteases (USPs) represent the most widespread and represented deubiquitinylating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
  USP2 is a ubiquitin-specific protease thought to function at the pre-proteasomal level by preventing the degradation of fatty acid synthase (FAS). FAS overexpression occurs in the majority of epithelial tumors, including prostate cancer, and protects cancer cells from apoptosis. Because USP2 is overexpressed in prostate cancer and its inhibition results in apoptosis of transformed prostatic cells, this isopeptidase is a potential target for induction of apoptosis in prostate cancer cells.
  The full length USP2 protein is comprised of 618 amino acids with a predicted molecular mass of 69.4 kDa and contains the highly conserved Cys and His boxes present in all members of the UBP family of deubiquitinylating enzymes. The N-terminal region (residues 271-618) of USP2 contains all the necessary residues for catalysis and exhibits deubiquitinylating activity vs. ubiquitin-AMC.
• Reconstitution and Storage: Store at -80C
• Purity: >95% (SDS-PAGE)
• Application Info: Uses: Deubiquitinylation assays using small molecule substrates (e.g. Ub-AMC) or ubiquitin-protein conjugates. Studies of the interaction of USP2 with FAS1 and their role in prostate cancer. Development of inhibitors for USP2 deubiquitinylating activity.

Target Info

• Reference: Broad utility of an affinity-enrichment strategy for unanchored polyubiquitin chains: J. Strachan, et al.; J. Proteomics Bioinform. S7, 001 (2013), Full Text
• Gene Symbol: Usp2
• Gene Full Name: ubiquitin specific peptidase 2
• Alias Symbols: 41 kDa ubiquitin-specific protease;deubiquitinating enzyme 2;ubiquitin carboxyl-terminal hydrolase 2;ubiquitin specific protease 2;ubiquitin thioesterase 2;ubiquitin thiolesterase 2;ubiquitin-specific-processing protease 2;ubiquitin-specific-processing protease testis;UBP-t.
• NCBI Gene Id: 115771
• Protein Name: Ubiquitin carboxyl-terminal hydrolase 2
• Description of Target: Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1 (By similarity). Isoform 1 and isoform 2 possess both ubiquitin-specific peptidase and isopeptidase activities (PubMed:12107281). Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity (By similarity). Has no deubiquitinase activity against p53/TP53 (By similarity). Prevents MDM2-mediated degradation of MDM4 (By similarity). Plays a role in the G1/S cell-cycle progression in normal and cancer cells (By similarity). Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues (PubMed:23213472). Associates with clock proteins and deubiquitinates core clock component PER1 but does not affect its overall stability (PubMed:23213472). Regulates the nucleocytoplasmic shuttling and nuclear retention of PER1 and its repressive role on the clock transcription factors CLOCK and ARNTL/BMAL1 (By similarity). Plays a role in the regulation of myogenic differentiation of embryonic muscle cells (PubMed:12107281).
• Uniprot ID: Q5U349
• Protein Size (# AA): Recombinant
• Molecular Weight: 40 kDa