USP15 Antibody (OAGA00329)

Catalog No: OAGA00329

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
OAGA00329 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
OAGA00329 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using USP15(OAGA00329) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using USP15(OAGA00329) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Ubiquitin (MIM 191339), a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). Also see USP1 (MIM 603478).[supplied by OMIM]
  Positive Controls: Molt-4, NIH-3T3, JC, BCL-1
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 920 and 981 of USP15 (Uniprot ID#Q9Y4E8)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.87 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: USP15
• Gene Full Name: Ubiquitin carboxyl-terminal hydrolase 15
• Alias Symbols: UNPH4, UNPH-2
• NCBI Gene Id: 9958
• Protein Name: Ubiquitin carboxyl-terminal hydrolase 15
• Uniprot ID: Q9Y4E8
• Protein Accession #: NP_001239007.1
• Molecular Weight: 109 kDa