UROD ELISA Kit (Rat) (OKCD01000)

Catalog No: OKCD01000 (Formerly GWB-KBBGM5)

Size:96WELLS

Rat Urod ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uroporphyrinogen Decarboxylase (UROD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uroporphyrinogen Decarboxylase (UROD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uroporphyrinogen Decarboxylase (UROD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Uroporphyrinogen Decarboxylase (UROD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uroporphyrinogen Decarboxylase (UROD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uroporphyrinogen Decarboxylase (UROD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Urod Microplate: 96 Wells (12 x 8 Well Strips)
  • Urod Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Urod Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.117 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Uroporphyrinogen Decarboxylase (UROD). No significant cross-reactivity or interference between Uroporphyrinogen Decarboxylase (UROD) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: UROD
• Gene Full Name: uroporphyrinogen decarboxylase
• Alias Symbols: porphyrinogen carboxy-lyase
• NCBI Gene Id: 29421
• Protein Name: Uroporphyrinogen decarboxylase
• Description of Target: Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.
• Uniprot ID: P32362
• Protein Accession #:  NP_062082
• Nucleotide Accession #: NM_019209.1
• Protein Size (# AA): 364