CAV1 ELISA Kit (Dog) (OKCA02052)

Catalog No: OKCA02052

Size:96 Wells

Dog CAV1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Dog
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	94	89-98
  Serum (n=4)	1:2	102	96-107
  Serum (n=4)	1:4	102	97-106
  Serum (n=4)	1:8	93	89-97

• ELISA Kit Principle: Aviva Systems Biology CAV1 ELISA Kit (Dog) (OKCA02052) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAV1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAV1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CAV1 captured in well.
• ELISA Kit Range: 0.625 ng/mL-40 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	97	90-102
  EDTA plasma (n=4)	98	92-103

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-CAV1 Microplate: 96 Wells (12 x 8 Well strips)
  • CAV1 Lyophilized Standard: 2 x 40 ng
  • 100X Biotinylated CAV1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates And Cell Lysates
• Sensitivity: 0.156 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CAV1
• NCBI Gene Id: 403980
• Protein Name: Caveolin-1
• Description of Target: Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation.
• Uniprot ID: P33724
• Protein Accession #: NP_001003296
• Nucleotide Accession #: NM_001003296