URAT1 ELISA Kit (Human) (OKCD04502)

Catalog No: OKCD04502

Size:96WELLS

Human URAT1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Urate Transporter 1 (URAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Urate Transporter 1 (URAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Urate Transporter 1 (URAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Urate Transporter 1 (URAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	85-94	89
  EDTA Plasma (n=5)	90-101	95
  Heparin Plasma (n=5)	87-101	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Urate Transporter 1 (URAT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Urate Transporter 1 (URAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ADH2 Microplate: 96 Wells (12 x 8 Well strips)
  • ADH2 Lyophilized Standard: 2
  • 100X ADH2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: 0.127 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SLC22A12
• Gene Full Name: solute carrier family 22 member 12
• Alias Symbols: OAT4L;organic anion transporter 4-like protein;renal-specific transporter;RST;solute carrier family 22 (organic anion/cation transporter), member 12;solute carrier family 22 (organic anion/urate transporter), member 12;solute carrier family 22 member 12;URAT1;urate anion exchanger 1;urate transporter 1.
• NCBI Gene Id: 116085
• Protein Name: Solute carrier family 22 member 12
• Description of Target: Required for efficient urate re-absorption in the kidney. Regulates blood urate levels. Mediates saturable urate uptake by facilitating the exchange of urate against organic anions.
• Uniprot ID: Q96S37
• Protein Accession #: NP_001263255.1
• Nucleotide Accession #: NM_001276326.1