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Catalog No: OKEH03414
Size:96T
Price: $525.00
SKU
OKEH03414
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology UPP1 ELISA Kit (Mouse) (OKEH03414) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Upp1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Upp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Upp1 captured in well.
ELISA Kit Range31.2-2000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.1% (n = 20)
Mean Inter-assay CV%: <= 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Upp1 Microplate96 Wells (12 x 8 Well strips)
Upp1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Upp1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity15.65 pg/mL
SpecificityNatural and recombinant Mouse Uridine phosphorylase 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolUpp1
Gene Full Nameuridine phosphorylase 1
Alias SymbolsAI325217;U;UdR;UdRPase;Up;UPase;UPase 1;Upp;urdPase 1;uridine phosphorylase 1.
NCBI Gene Id22271
Protein NameP52624
Description of TargetUridine phosphorylase 1
Uniprot IDhttps://www.uniprot.org/uniprot/P52624
Protein Accession #NP_001152873.1
Nucleotide Accession #NM_001159401.1
Protein Size (# AA)311
Molecular Weight34 kDa
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