UPP1 ELISA Kit (Mouse) (OKCD07684)

Catalog No: OKCD07684

Size:96WELLS

UPP1 ELISA Kit (Mouse) (OKCD07684) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uridine Phosphorylase 1 (UPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uridine Phosphorylase 1 (UPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uridine Phosphorylase 1 (UPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Uridine Phosphorylase 1 (UPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uridine Phosphorylase 1 (UPP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uridine Phosphorylase 1 (UPP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • UPP1 Microplate: 96 Wells (12 x 8 Well strips)
  • UPP1 Lyophilized Standard: 2
  • 100X UPP1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.055ng/mL

Target Info

• Gene Symbol: Upp1
• Gene Full Name: uridine phosphorylase 1
• Alias Symbols: AI325217;U;UdR;UdRPase;Up;UPase;UPase 1;Upp;urdPase 1;uridine phosphorylase 1.
• NCBI Gene Id: 22271
• Protein Name: Uridine phosphorylase 1
• Description of Target: Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate (PubMed:7744869). The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.
• Uniprot ID: P52624
• Protein Accession #: NP_001152873.1
• Nucleotide Accession #: NM_001159401.1