UPK3A ELISA Kit (Human) (OKCD00978)

Catalog No: OKCD00978 (Formerly GWB-KBBKK4)

Size:96WELLS

Human UPK3A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	81-103%	97-105%	94-105%	98-105%
  EDTA plasma(n=5)	91-98%	81-104%	81-101%	78-104%
  heparin plasma(n=5)	78-105%	79-92%	98-105%	78-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uroplakin 3A (UPK3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uroplakin 3A (UPK3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uroplakin 3A (UPK3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Uroplakin 3A (UPK3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-97	86
  EDTA plasma(n=5)	80-90	85
  heparin plasma(n=5)	79-101	83

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uroplakin 3A (UPK3A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uroplakin 3A (UPK3A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-UPK3A Microplate: 96 Wells (12 x 8 Well Strips)
  • UPK3A Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated UPK3A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.125 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Uroplakin 3A (UPK3A). No significant cross-reactivity or interference between Uroplakin 3A (UPK3A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: UPK3A
• Gene Full Name: uroplakin 3A
• Alias Symbols: UP3A;UPIII;UPIIIA;UPK3;uroplakin 3;uroplakin III;uroplakin-3a.
• NCBI Gene Id: 7380
• Protein Name: Uroplakin-3a
• Description of Target: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence (By similarity).
• Uniprot ID: O75631
• Protein Accession #: NP_001123979
• Nucleotide Accession #: NM_001130507.1
• Protein Size (# AA): 287