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Datasheets/Manuals | Printable datasheet for anti-UPF1 (ARP89425_P050) antibody |
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Tested Species Reactivity | Mouse |
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Predicted Species Reactivity | Mouse |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the C terminal region of mouse UPF1 |
Purification | Affinity purified |
Peptide Sequence | Synthetic peptide located within the following region: GQANGPAAGRGTPKTKTGRGGRQKNRFGLPGPSQTTLPNSQASQDVASQP |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-UPF1 (ARP89425_P050) antibody is Catalog # AAP89425 |
Gene Symbol | UPF1 |
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Gene Full Name | UPF1 regulator of nonsense transcripts homolog (yeast) |
Alias Symbols | PNO, Ren, NORF1, Rent1, Upflp, PNORF-1, B430202H16Rik |
NCBI Gene Id | 19704 |
Protein Name | regulator of nonsense transcripts 1 |
Description of Target | RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD (By similarity). Essential for embryonic viability. |
Uniprot ID | Q9EPU0 |
Protein Accession # | NP_001116301.1 |
Nucleotide Accession # | NM_001122829.1 |
Protein Size (# AA) | 1124 |
Molecular Weight | 123 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "UPF1 Antibody - C-terminal region (ARP89425_P050)"?
The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "Mouse".
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How long will it take to receive "UPF1 Antibody - C-terminal region (ARP89425_P050)"?
This item is available "Domestic: within 24 hours delivery | International: 3-5 days".
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What buffer format is "UPF1 Antibody - C-terminal region (ARP89425_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "UPF1 Antibody - C-terminal region (ARP89425_P050)"?
This target may also be called "PNO, Ren, NORF1, Rent1, Upflp, PNORF-1, B430202H16Rik" in publications.
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What is the shipping cost for "UPF1 Antibody - C-terminal region (ARP89425_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "UPF1 Antibody - C-terminal region (ARP89425_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "UPF1 Antibody - C-terminal region (ARP89425_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "123 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "UPF1 Antibody - C-terminal region (ARP89425_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "UPF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "UPF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "UPF1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "UPF1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "UPF1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "UPF1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.