ULK2 Antibody - C-terminal region (OAAB17470)

Catalog No: OAAB17470

Size:400ul

Western blot analysis of ULK2 Antibody (C-term) (Cat.# OAAB17470) in mouse brain tissue lysates (35ug/lane). ULK2(arrow) was detected using the purified Pab.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
• Additional Information: Precautions: ULK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons
  Cellular Location: Cytoplasmic vesicle membrane; Peripheral membrane protein. Note=Localizes to pre-autophagosomal membrane
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ULK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 612-643 amino acids from the C-terminal region of human ULK2.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000

Target Info

• Gene Symbol: ULK2
• Alias Symbols: ATG1B, Unc51.2
• NCBI Gene Id: 9706
• Protein Name: Serine/threonine-protein kinase ULK2
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q8IYT8
• Protein Accession #: NP_001136082.1
• Molecular Weight: 112694 Da