ULK1 Antibody (Phospho-Ser317) (OAAB16352)

Catalog No: OAAB16352

Size:400ul

Dot blot analysis of ULK1 Antibody (Phospho S317) Phospho-specific Pab (Cat. # OAAB16352) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: DB
• Additional Information: Function: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy.
  Cellular Location: Cytoplasm, cytosol. Preautophagosomal structure. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  Tissue Location: Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ULK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S317 of human ULK1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.5 mg/ml
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: DB: 1:500
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: ULK1
• Alias Symbols: ATG1, ATG1A, UNC51, hATG1, Unc51.1
• NCBI Gene Id: 8408
• Protein Name: Serine/threonine-protein kinase ULK1
• Description of Target: Involved in autophagy. Required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Phosphorylates ATG13/KIAA0652. Involved in axon growth. Plays an essential role in neurite extension of cerebellar granule cells.
• Uniprot ID: O75385
• Protein Accession #: NP_003556.1
• Molecular Weight: 113 kDa