SAVE NOW with 10% off all Recombinant Antibodies Shop Now

Catalog No: OKWB00203
Size:96T
Price: $450.00
SKU
OKWB00203
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for ULK1 ELISA Kit (Human) (OKWB00203)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)88-104%86-103%88-104%88-98%
EDTA Plasma (n=5)84-95%82-101%84-92%85-101%
Heparin Plasma (n=5)80-99%81-98%81-97%81-99%
ELISA Kit PrincipleAviva Systems Biology ULK1 ELISA Kit (Human ) (OKWB00203) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ULK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ULK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ULK1 captured in the well.
ELISA Kit Range15.625-1000pg/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)86-9792
EDTA Plasma (n=5)90-10598
Heparin Plasma (n=5)87-10396
ELISA Kit ReproducibilityMean Intra-Assay Precision < 8%
Mean Inter-Assay Precision < 10%
ELISA Kit Component
ComponentAmount
ULK1 Microplate96 Wells (12 x 8 Well strips)
ULK1 Lyophilized Standard2 x 1 ng
100X ULK1 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates and Other Biological Fluids
Sensitivity< 9.375
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolULK1
Gene Full Nameunc-51 like autophagy activating kinase 1
Alias SymbolsATG1;ATG1 autophagy related 1 homolog;ATG1A;autophagy-related protein 1 homolog;hATG1;serine/threonine-protein kinase ULK1;UNC51;Unc51.1;Unc-51-like kinase 1.
NCBI Gene Id8408
Protein NameSerine/threonine-protein kinase ULK1
Description of TargetSerine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
Uniprot IDO75385
Protein Accession #NP_003556
Nucleotide Accession #NM_003565
Write Your Own Review
You're reviewing:ULK1 ELISA Kit (Human) (OKWB00203)
Your Rating
We found other products you might like!