ULK1 Antibody (Phospho-Ser556) (OABF01248)

Catalog No: OABF01248 (Formerly GWB-F0FWT6)

Size:100ul

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-ATG1 (Ser556) Polyclonal Antibody, Unconjugated (OABF01248) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC-P, Fluorescent-IHC
• Additional Information: Subcellular location: Cytoplasm
  
• Reconstitution and Storage: Store at -20C for 12 months.
• Immunogen: KLH conjugated synthetic phosphopeptide derived from human ATG1 around the phosphorylation site of Ser556, between 530-580 amino acids.
• Purification: Purified by Protein A.
• Concentration: 1ug/ul
• Warning: This product contains sodium azide, which forms hydrazoic acid under acidic conditions and may react with lead or copper plumbing to produce explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Intended Use: For research use only. Not intended for diagnostic or therapeutic use.
• Application Info: WB: 1:100~1000
  IHC-P: 1:100~500
  Fluorescent-IHC: 1:50~200
• Publications:

  Mir214-3p and Hnf4a/Hnf4α reciprocally regulate Ulk1 expression and autophagy in nonalcoholic hepatic steatosis. Autophagy. NULL, 1-17 (2020). 33078654

  The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples. Nutrients. 12, (2020). 32121154

Target Info

• Gene Symbol: ULK1
• Gene Full Name: unc-51 like autophagy activating kinase 1
• Alias Symbols: ATG1, ATG1A, UNC51, hATG1, Unc51.1
• NCBI Gene Id: 8408
• Description of Target: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.
• Uniprot ID: O75385