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Catalog No: OKEH03938
Size:96T
Price: $625.00
SKU
OKEH03938
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology UCP2 ELISA Kit (Bovine) (OKEH03938) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UCP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UCP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UCP2 captured in well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit Component
ComponentAmount
UCP2 Microplate96 Wells (12 x 8 Well strips)
UCP2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated UCP2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.095 ng/mL
SpecificityNatural and recombinant Bovine Mitochondrial uncoupling protein 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolUCP2
Gene Full Nameuncoupling protein 2
Alias Symbolsmitochondrial uncoupling protein 2;solute carrier family 25 member 8;UCP 2;uncoupling protein 2 (mitochondrial, proton carrier).
NCBI Gene Id281562
Protein NameQ3SZI5
Description of TargetMitochondrial uncoupling protein 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q3SZI5
Protein Accession #NP_001028783.1
Nucleotide Accession #NM_001033611.2
Protein Size (# AA)309
Molecular Weight309 kDa
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