Catalog No: OKCD08811
Size:96WELLS
Price: $850.00
SKU
OKCD08811
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for UCP1 ELISA Kit (Rat) (OKCD08811) |
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Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uncoupling Protein 1, Mitochondrial (UCP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uncoupling Protein 1, Mitochondrial (UCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uncoupling Protein 1, Mitochondrial (UCP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Uncoupling Protein 1, Mitochondrial (UCP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uncoupling Protein 1, Mitochondrial (UCP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uncoupling Protein 1, Mitochondrial (UCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.119ng/mL |
Gene Symbol | Ucp1 |
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Gene Full Name | uncoupling protein 1 |
Alias Symbols | mitochondrial brown fat uncoupling protein 1;mitochondrial brown fat uncoupling protein 1-like;solute carrier family 25 member 7;thermogenin;Ucp;UCP 1;Ucpa;uncoupling protein 1 (mitochondrial, proton carrier);Uncp. |
NCBI Gene Id | 24860 |
Protein Name | Mitochondrial brown fat uncoupling protein 1 |
Description of Target | Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates to non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (By similarity). Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs (PubMed:12479871, PubMed:16814247). Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria (By similarity). |
Uniprot ID | P04633 |
Protein Accession # | NP_036814.1 |
Nucleotide Accession # | NM_012682.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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