UCP1 ELISA Kit (Rat) (OKCA01905)

Catalog No: OKCA01905

Size:96 Wells

Rat UCP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution

  Mean Recovery (%)

  Range (%)

• ELISA Kit Principle: Aviva Systems Biology UCP1 ELISA Kit (Rat) (OKCA01905) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UCP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UCP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample UCP1 captured in the well.
• ELISA Kit Range: 31.25 - 2,000 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	91	87-95
  EDTA Plasma (n=4)	98	94-102

• ELISA Kit Component:
  • UCP1 Microplate: 96 Wells (12 x 8 Well strips)
  • UCP1 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated UCP1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 7.81 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: UCP1
• Gene Full Name: Mitochondrial brown fat uncoupling protein 1
• Alias Symbols: SLC25A7, UCP, mitochondrial brown fat uncoupling protein|thermogenin|uncoupling protein 1
• NCBI Gene Id: 100328618
• Protein Name: Mitochondrial brown fat uncoupling protein 1
• Description of Target: Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates to non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance. Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria.
• Uniprot ID: P14271
• Protein Accession #: NP_001164548.1
• Nucleotide Accession #: NM_001171077.1