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Catalog No: OKCD08810
Size:96WELLS
Price: $800.00
SKU
OKCD08810
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Datasheets/ManualsPrintable datasheet for UCP1 ELISA Kit (Human) (OKCD08810)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uncoupling Protein 1, Mitochondrial (UCP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uncoupling Protein 1, Mitochondrial (UCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uncoupling Protein 1, Mitochondrial (UCP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Uncoupling Protein 1, Mitochondrial (UCP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uncoupling Protein 1, Mitochondrial (UCP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uncoupling Protein 1, Mitochondrial (UCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
UCP1 Microplate96 Wells (12 x 8 Well strips)
UCP1 Lyophilized Standard2
100X UCP1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.064ng/mL
Gene SymbolUCP1
Gene Full Nameuncoupling protein 1
Alias Symbolsmitochondrial brown fat uncoupling protein 1;SLC25A7;solute carrier family 25 member 7;thermogenin;UCP;uncoupling protein 1 (mitochondrial, proton carrier).
NCBI Gene Id7350
Protein NameMitochondrial brown fat uncoupling protein 1
Description of TargetMitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates to non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (By similarity). Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane (PubMed:24196960). However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria (By similarity).
Uniprot IDP25874
Protein Accession #NP_068605.1
Nucleotide Accession #NM_021833.4
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