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Catalog No: OKEH01465
Size:96T
Price: $600.00
SKU
OKEH01465
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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UCP1 ELISA Kit (Human) : 96 Wells (OKEH01465)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology UCP1 ELISA Kit (Human) (OKEH01465) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UCP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UCP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UCP1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 8.5% (n = 20)
ELISA Kit Component
ComponentAmount
UCP1 Microplate96 Wells (12 x 8 Well strips)
UCP1 Lyophilized Standard2
100X Biotinylated UCP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity28 pg/mL
SpecificityNatural and recombinant Human Mitochondrial brown fat uncoupling protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolUCP1
Gene Full Nameuncoupling protein 1
Alias Symbolsmitochondrial brown fat uncoupling protein 1;SLC25A7;solute carrier family 25 member 7;thermogenin;UCP;uncoupling protein 1 (mitochondrial, proton carrier).
NCBI Gene Id7350
Protein NameP25874
Description of TargetMitochondrial brown fat uncoupling protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/P25874
Protein Accession #NP_068605.1
Nucleotide Accession #NM_021833.4
Protein Size (# AA)307
Molecular Weight33 kDa
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