UCHL4 ELISA Kit (Mouse) (OKCD09497)

Catalog No: OKCD09497

Size:96WELLS

UCHL4 ELISA Kit (Mouse) (OKCD09497) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L4 (UCHL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L4 (UCHL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Carboxyl Terminal Hydrolase L4 (UCHL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ubiquitin Carboxyl Terminal Hydrolase L4 (UCHL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L4 (UCHL4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L4 (UCHL4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • UCHL4 Microplate: 96 Wells (12 x 8 Well strips)
  • UCHL4 Lyophilized Standard: 2
  • 100X UCHL4 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.138ng/mL

Target Info

• Gene Symbol: Uchl4
• Gene Full Name: ubiquitin carboxyl-terminal esterase L4
• Alias Symbols: ubiquitin carboxyl-terminal hydrolase isozyme L4;ubiquitin thioesterase L4;UCH-L4.
• NCBI Gene Id: 93841
• Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L4
• Description of Target: Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.
• Uniprot ID: P58321
• Protein Accession #: NP_291085.1
• Nucleotide Accession #: NM_033607.1