UBR4 ELISA Kit (Human) : 96 Wells (OKEH00289)

Catalog No: OKEH00289

Size:96T

Human UBR4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology UBR4 ELISA Kit (Human) (OKEH00289) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human UBR4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated UBR4 and incubated. The UBR4 found in the sample or standards competes with the biotinylated UBR4 for limited binding sites on the immobilized anti-human UBR4 antibody. Excess unbound biotinylated UBR4 and sample or standard UBR4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated UBR4 captured in the well and inversely proportional to the amount of UBR4 which was contained in the sample or standard.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.6% (n = 20)
  Mean Inter-assay CV%: < 7.8% (n = 20)
• ELISA Kit Component:
  • Anti-UBR4 Microplate: 96 Wells (12 x 8 Well strips)
  • UBR4 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X UBR4-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.22 ng/mL
• Specificity: Natural and recombinant Human E3 ubiquitin-protein ligase UBR4
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: UBR4
• Gene Full Name: ubiquitin protein ligase E3 component n-recognin 4
• Alias Symbols: 600 kDa retinoblastoma protein-associated factor;E3 ubiquitin-protein ligase UBR4;N-recognin-4;p600;RBAF600;retinoblastoma-associated factor 600-like protein;retinoblastoma-associated factor of 600 kDa;RING-type E3 ubiquitin transferase UBR4;zinc finger UBR1-type protein 1;ZUBR1.
• NCBI Gene Id: 23352
• Protein Name: Q5T4S7
• Description of Target: E3 ubiquitin-protein ligase UBR4
• Uniprot ID: https://www.uniprot.org/uniprot/Q5T4S7
• Protein Accession #: NP_065816.2
• Nucleotide Accession #: NM_020765.2
• Protein Size (# AA): 5183
• Molecular Weight: 573 kDa