UBP1 Antibody - middle region (OAAB10363)

Catalog No: OAAB10363

Size:400ul

UBP1 Antibody (Center) (Cat. #OAAB10363) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the UBP1 antibody detected the UBP1 protein (arrow).

UBP1 Antibody (Center) (OAAB10363)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Fluorescent image of A549 cell stained with UBP1 Antibody (Center)(Cat#OAAB10363).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UBP1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).UBP1 immunoreactivity is localized to Nucleus significantly.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, IF
  Cellular Location: Nucleus.
  Tissue Location: Expressed in adrenal tissue, JEG-3, NCI-H295A, Hep-G2 and HeLa cell lines
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This UBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human UBP1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  UBP1 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Application Info: IF: 1:10~50
  WB: 1:1000
  IHC-P: 1:10~50

Target Info

• Gene Symbol: UBP1
• Alias Symbols: LBP1A, LBP1B, LBP-1B, LBP-1a
• NCBI Gene Id: 7342
• Protein Name: Upstream-binding protein 1
• Description of Target: UBP1 functions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2 (By similarity). Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.
• Uniprot ID: Q9NZI7
• Protein Accession #: NP_001121632.1
• Molecular Weight: 60 kDa