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Catalog No: OASE00106 (Formerly GWB-F84519)
Size:50ug
Price: $219.00
SKU
OASE00106
Availability: Discontinued
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Ubiquitin Antibody (OASE00106)

Datasheets/ManualsPrintable datasheet for OASE00106
Product Info
Predicted Species ReactivityHuman, Mouse, Rat, Bovine
ClonalityMonoclonal
Clone6C11-B3
IsotypeIgG2a Kappa
HostMouse
ConjugationUnconjugated
ApplicationWB, ICC, IF, ELISA
Additional InformationBackground Info: This antibody recognizes ~10kDa corresponding to free ubiquitin
::Scientific Background: Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an
ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(2, 3). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (4). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as I?Balpha and p27 (5).
::Certificate of Analysis: 1 ug/mL was sufficient for detection of ubiquitin in 10ug of Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Reconstitution and StorageStore at -20C. Shipping with Blue Ice or 4C.
ImmunogenNative bovine ubiquitin, conjugated to KLH
PurificationProtein G Purified
Concentration1 mg/ml
SpecificityDetects ~10kDa.
DilutionWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Storage BufferPBS pH7.4, 50% glycerol, 0.09% sodium azide
Reference1. Wilkinson K.D. (1995) Annu. Rev. Nutr. 15:161-189. 2. Bonifacino J.S., et al. (1998) Annu Rev Cell Dev Biol. 14:
19-57.
3. Boston Biochem: "Ubiquitin Proteasome Pathway Overview” //www.bostonbiochem.com/upp.php
4. Yang M., et al. (1998) J Exp Med. 187: 1835-1846.
5. Chen Z.J., et al. (1996) Cell 84: 853-862.
DescriptionUbiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(2, 3). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (4). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (5).
Gene SymbolUbiquitin
Gene Full Nameubiquitin B
Alias SymbolsPolyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B
NCBI Gene Id281370
Uniprot IDP0CG53
Protein Accession #NP_776558.1
  1. What is the species homology for "Ubiquitin Antibody (OASE00106)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Bovine".

  2. How long will it take to receive "Ubiquitin Antibody (OASE00106)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Ubiquitin Antibody (OASE00106)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Ubiquitin Antibody (OASE00106)"?

    This target may also be called "Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B" in publications.

  5. What is the shipping cost for "Ubiquitin Antibody (OASE00106)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Ubiquitin Antibody (OASE00106)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Ubiquitin Antibody (OASE00106)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Ubiquitin Antibody (OASE00106)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "Ubiquitin"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "Ubiquitin"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "Ubiquitin"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "Ubiquitin"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "Ubiquitin"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "Ubiquitin"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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