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Datasheets/Manuals | Printable datasheet for OAAB08556 |
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Tested Species Reactivity | Human |
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Product Format | Liquid. PBS with 0.09% (W/V) sodium azide. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB, IHC-P, IF |
Additional Information | Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling Cellular Location: Ubiquitin: Cytoplasm. Nucleus |
Reconstitution and Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This Ubiquitin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-66 amino acids of human Ubiquitin. |
Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Predicted Homology Based on Immunogen Sequence | Yeast, Bovine, C. Elegans, Drosophila, Monkey, Mouse, Pig, Rat, Sheep, Chicken, Horse, Hamster, Xenopus, Rabbit |
Concentration | 1.9 mg/ml (lot specific) |
Intended Use | Ubiquitin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Application Info | WB: 1:1000 IHC-P: 1:50~100 IF: 1:10~50 |
Gene Symbol | UBC |
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Alias Symbols | UBC, Polyubiquitin-C, Ubiquitin |
Protein Name | Polyubiquitin-C |
Description of Target | This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. |
Uniprot ID | P0CG48 |
Molecular Weight | 77 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Ubiquitin Antibody - C-terminal region (OAAB08556)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "".
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How long will it take to receive "Ubiquitin Antibody - C-terminal region (OAAB08556)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "Ubiquitin Antibody - C-terminal region (OAAB08556)" provided in?
This item is provided in "Liquid. PBS with 0.09% (W/V) sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Ubiquitin Antibody - C-terminal region (OAAB08556)"?
This target may also be called "UBC, Polyubiquitin-C, Ubiquitin" in publications.
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What is the shipping cost for "Ubiquitin Antibody - C-terminal region (OAAB08556)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Ubiquitin Antibody - C-terminal region (OAAB08556)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Ubiquitin Antibody - C-terminal region (OAAB08556)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "77 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Ubiquitin Antibody - C-terminal region (OAAB08556)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "UBC"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "UBC"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "UBC"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "UBC"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "UBC"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "UBC"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.