UBE2R2 Antibody (OAGA05734)

Catalog No: OAGA05734

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Jurkat
12% SDS PAGE
diluted at 1:5000

Sample (50 ug of whole cell lysate)
A: mouse liver
12% SDS PAGE
diluted at 1:1000

UBC3B antibody detects UBC3B protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UBC3B protein stained by UBC3B antibody diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human UBC3B. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.69 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: UBE2R2
• Gene Full Name: ubiquitin conjugating enzyme E2 R2
• Alias Symbols: UBC3B, CDC34B, E2-CDC34B
• NCBI Gene Id: 54926
• Protein Name: ubiquitin-conjugating enzyme E2 R2
• Description of Target: Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation.
• Uniprot ID: Q712K3
• Protein Accession #: NP_060281.2
• Nucleotide Accession #: NM_017811.3