UBE2I ELISA Kit (Human) (OKEH02141)

Catalog No: OKEH02141

Size:96T

Human UBE2I ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology UBE2I ELISA Kit (Human) (OKEH02141) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UBE2I has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UBE2I is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UBE2I captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.7% (n = 20)
  Mean Inter-assay CV%: < 8.3% (n = 20)
• ELISA Kit Component:
  • UBE2I Microplate: 96 Wells (12 x 8 Well strips)
  • UBE2I Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated UBE2I Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Human SUMO-conjugating enzyme UBC9
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: UBE2I
• Gene Full Name: ubiquitin conjugating enzyme E2 I
• Alias Symbols: C358B7.1;P18;RING-type E3 SUMO transferase UBC9;SUMO-1-protein ligase;SUMO-conjugating enzyme UBC9;SUMO-protein ligase;UBC9;ubiquitin carrier protein 9;ubiquitin carrier protein I;ubiquitin conjugating enzyme 9;ubiquitin conjugating enzyme E2I;Ubiquitin-conjugating enzyme E2 I;ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9);ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast);ubiquitin-conjugating enzyme UbcE2A;ubiquitin-like protein SUMO-1 conjugating enzyme;ubiquitin-protein ligase E2I;ubiquitin-protein ligase I.
• NCBI Gene Id: 7329
• Protein Name: P63279
• Description of Target: SUMO-conjugating enzyme UBC9
• Uniprot ID: https://www.uniprot.org/uniprot/P63279
• Protein Accession #: NP_003336.1
• Nucleotide Accession #: NM_003345.4
• Protein Size (# AA): 158
• Molecular Weight: 18 kDa