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Datasheets/Manuals | Printable datasheet for UBE2I Antibody (OAEB02456) |
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Predicted Species Reactivity | Dog|Human|Mouse|Rat |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB, IHC |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | UBE2I |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Human: 100%; Mouse: 100% |
Peptide Sequence | SGIALSRLAQERK-C |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx 17kDa band observed in Human Kidney, Human Lung and Human Testis lysates (calculated MW of 18kDa according to NP_003336). Recommended concentration: 0.1-0.3ug/ml Immunohistochemistry: In paraffin embedded Human Kidney, shows nuclear staining of some epithelial cells of glomeruli and renal tubules. Recommended concentration, 1ug/ml. |
Reference | * Ihara M, Stein P, Schultz RM.; UBE2I (UBC9), a SUMO-conjugating enzyme, localizes to nuclear speckles and stimulates transcription in mouse oocytes.; Biol Reprod. 2008 Nov;79(5):906-13.; Pubmed 18703419 * Wang ZY, Qiu QQ, Seufert W, Taguchi T, Testa JR, Whitmore SA, Callen DF, Welsh D, Shenk T, Deuel TF.; Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9.; J Biol Chem. 1996 Oct 4;271(40):24811-6.; Pubmed 8798754 |
Gene Symbol | Ube2i|UBE2I |
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Gene Full Name | ubiquitin conjugating enzyme E2 I|ubiquitin-conjugating enzyme E2I |
Alias Symbols | 5830467E05Rik;C358B7.1;P18;RING-type E3 SUMO transferase UBC9;SUMO-1-protein ligase;SUMO-conjugating enzyme UBC9;SUMO-protein ligase;UBC9;UbcE2A;Ubce2i;Ubce9;ubiquitin carrier protein 9;ubiquitin carrier protein I;ubiquitin conjugating enzyme 9;ubiquitin conjugating enzyme E2I;Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9);ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9);ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast);ubiquitin-conjugating enzyme UbcE2A;ubiquitin-like protein SUMO-1 conjugating enzyme;ubiquitin-protein ligase E2I;ubiquitin-protein ligase I. |
NCBI Gene Id | 22196|25573|7329 |
Protein Name | SUMO-conjugating enzyme UBC9 |
Uniprot ID | P63279 |
Protein Accession # | NP_003336.1 |
Molecular Weight | 18.0; 18.0; 18.0; 18.0 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "UBE2I Antibody (OAEB02456)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Dog|Human|Mouse|Rat".
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How long will it take to receive "UBE2I Antibody (OAEB02456)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "UBE2I Antibody (OAEB02456)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "UBE2I Antibody (OAEB02456)"?
This target may also be called "5830467E05Rik;C358B7.1;P18;RING-type E3 SUMO transferase UBC9;SUMO-1-protein ligase;SUMO-conjugating enzyme UBC9;SUMO-protein ligase;UBC9;UbcE2A;Ubce2i;Ubce9;ubiquitin carrier protein 9;ubiquitin carrier protein I;ubiquitin conjugating enzyme 9;ubiquitin conjugating enzyme E2I;Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9);ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9);ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast);ubiquitin-conjugating enzyme UbcE2A;ubiquitin-like protein SUMO-1 conjugating enzyme;ubiquitin-protein ligase E2I;ubiquitin-protein ligase I." in publications.
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What is the shipping cost for "UBE2I Antibody (OAEB02456)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "UBE2I Antibody (OAEB02456)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "UBE2I Antibody (OAEB02456)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "18.0; 18.0; 18.0; 18.0".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "UBE2I Antibody (OAEB02456)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Ube2i|UBE2I"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Ube2i|UBE2I"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Ube2i|UBE2I"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Ube2i|UBE2I"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Ube2i|UBE2I"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Ube2i|UBE2I"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.