UBE2I Antibody (OAEB02456)

Catalog No: OAEB02456 (Formerly GWB-57B82B)

Size:100UG

OAEB02456 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02456 (0.3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: UBE2I
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Human: 100%; Mouse: 100%
• Peptide Sequence: SGIALSRLAQERK-C
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:64000.
  Western Blot: Approx 17kDa band observed in Human Kidney, Human Lung and Human Testis lysates (calculated MW of 18kDa according to NP_003336). Recommended concentration: 0.1-0.3ug/ml
  Immunohistochemistry: In paraffin embedded Human Kidney, shows nuclear staining of some epithelial cells of glomeruli and renal tubules. Recommended concentration, 1ug/ml.
• Reference: * Ihara M, Stein P, Schultz RM.; UBE2I (UBC9), a SUMO-conjugating enzyme, localizes to nuclear speckles and stimulates transcription in mouse oocytes.; Biol Reprod. 2008 Nov;79(5):906-13.; Pubmed 18703419
  * Wang ZY, Qiu QQ, Seufert W, Taguchi T, Testa JR, Whitmore SA, Callen DF, Welsh D, Shenk T, Deuel TF.; Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9.; J Biol Chem. 1996 Oct 4;271(40):24811-6.; Pubmed 8798754

Target Info

• Gene Symbol: Ube2i|UBE2I
• Gene Full Name: ubiquitin conjugating enzyme E2 I|ubiquitin-conjugating enzyme E2I
• Alias Symbols: 5830467E05Rik;C358B7.1;P18;RING-type E3 SUMO transferase UBC9;SUMO-1-protein ligase;SUMO-conjugating enzyme UBC9;SUMO-protein ligase;UBC9;UbcE2A;Ubce2i;Ubce9;ubiquitin carrier protein 9;ubiquitin carrier protein I;ubiquitin conjugating enzyme 9;ubiquitin conjugating enzyme E2I;Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9);ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9);ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast);ubiquitin-conjugating enzyme UbcE2A;ubiquitin-like protein SUMO-1 conjugating enzyme;ubiquitin-protein ligase E2I;ubiquitin-protein ligase I.
• NCBI Gene Id: 22196|25573|7329
• Protein Name: SUMO-conjugating enzyme UBC9
• Uniprot ID: P63279
• Protein Accession #: NP_003336.1
• Molecular Weight: 18.0; 18.0; 18.0; 18.0