UBC Antibody (OAAB21995)

Catalog No: OAAB21995

Size:100ul

Ubiquitin protein probed with Ubiquitin Antibody, Unconjugated (OAAB21995) at 1:300 overnight at 4C. Followed by a conjugated secondary antibody at 1:5,000 for 90 min at 37C.

Formalin-fixed and paraffin embedded human breast cancer labeled with Ubiquitin Antibody, Unconjugated (OAAB21995) followed by conjugation to the secondary antibody and DAB staining

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IF, IHC-P
• Additional Information: Function: Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.03% Proclin300 and 50% glycerol
• Formulation: 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
• Application Info: IHC-P: 1:100~1:500
  WB: 1:100~1:500

Target Info

• Gene Symbol: UBC
• Gene Full Name: ubiquitin C
• Alias Symbols: HMG20
• NCBI Gene Id: 7316
• Description of Target: This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways.
• Uniprot ID: P0CG48
• Protein Accession #: NP_066289.3
• Nucleotide Accession #: NM_021009.6
• Molecular Weight: 77 kDa