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Catalog No: OKEH04416
Size:96T
Price: $525.00
SKU
OKEH04416
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology UBA3 ELISA Kit (Human) (OKEH04416) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UBA3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for UBA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UBA3 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.7% (n = 20)
Mean Inter-assay CV%: <= 9.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-UBA3 Microplate96 Wells (12 x 8 Well Strips)
UBA3 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
CXCL6 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.081 ng/mL
SpecificityNatural and recombinant Human NEDD8-activating enzyme E1 catalytic subunit
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolUBA3
Gene Full Nameubiquitin like modifier activating enzyme 3
Alias SymbolshUBA3;NAE2;NEDD8-activating enzyme E1 catalytic subunit;NEDD8-activating enzyme E1 subunit 2;NEDD8-activating enzyme E1C;Nedd8-activating enzyme hUba3;UBA3, ubiquitin-activating enzyme E1 homolog;UBE1C;ubiquitin-activating enzyme 3;Ubiquitin-activating enzyme E1C;ubiquitin-activating enzyme E1C (homologous to yeast UBA3);ubiquitin-activating enzyme E1C (UBA3 homolog, yeast);Ubiquitin-like modifier-activating enzyme 3.
NCBI Gene Id9039
Protein NameQ8TBC4
Description of TargetNEDD8-activating enzyme E1 catalytic subunit
Uniprot IDhttps://www.uniprot.org/uniprot/Q8TBC4
Protein Accession #NP_003959.3
Nucleotide Accession #NM_003968.3
Protein Size (# AA)463
Molecular Weight52 KDa
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