UBA1 Recombinant Protein (Human) (OPED00157)

Catalog No: OPED00157

Size:50UG

Figure A: Product purity determined by Coomassie staining following SDS-PAGE Figure B: Western Blot of thioester assays (TE +ve/-ve controls) for E2 conjugating enzymes. Biotinylated-ubiquitin-enzyme conjugates were detected by western blotting on thioester assays containing: A: UbcH2 , B: UbcH8 (KW9135), respectively, using Streptavidin-HRP detection system. M: Biotinylated SDS molecular weight markers (Sigma, SDS-6B) from bottom: 20.1, 29.0, 39.8, 58.1kDa.

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: In 50mM TRIS-hydrochloric acid, containing 1.0mM dithiothreitol
• Host: E. Coli
• Additional Information: Results demonstrate the formation of ubiquitin thioester linked E2 conjugates of the expected size in all TE +ve control reactions. The absence of such conjugates in TE –ve control reactions demonstrates that their formation is ATP dependent (required for E1 activation) and hence derived from the ubiquitin cascade.
• Reconstitution and Storage: Store at -80C
• Purification: Purified by multi-step chromatography.
• Purity: >90% (SDS-PAGE)
• Reference: F-box protein Fbxl18 mediates polyubiquitylation and proteasomal degradation of the pro-apoptotic SCF subunit Fbxl7: Y. Liu, et al.; Cell Death Dis. 6, e1630 (2015), Application(s): Assay, Abstract; Full Text
  Pro-apoptotic F-box protein Fbxl7 regulates mitochondrial function by mediating the ubiquitylation and proteasomal degradation of survivin: Y. Liu, et al.; J. Biol. Chem. 290, 11843 (2015), Application(s): Assay, Abstract; Full Text
  UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP: Y. Teng, et al.; Biochem. Pharmacol. 97, 518 (2015), Application(s): Western Blot, Abstract;
  Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation: A. L. Haas, et al.; J. Biol. Chem. 257, 2543 (1982), Abstract;
  Activation of the heat-stable polypeptide of the ATP-dependent proteolytic system: A. L. Cienchanover; PNAS 78, 761 (1981), Abstract;

Target Info

• Gene Symbol: UBA1
• Gene Full Name: ubiquitin like modifier activating enzyme 1
• Alias Symbols: A1S9;A1S9T;A1S9T and BN75 temperature sensitivity complementing;A1ST;AMCX1;CFAP124;GXP1;POC20;POC20 centriolar protein homolog;Protein A1S9;SMAX2;testicular secretory protein Li 63;UBA1, ubiquitin-activating enzyme E1 homolog A;UBA1A;UBE1;UBE1X;Ubiquitin-activating enzyme E1;ubiquitin-like modifier-activating enzyme 1;VEXAS.
• NCBI Gene Id: 7317
• Protein Name: Ubiquitin-like modifier-activating enzyme 1
• Description of Target: Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).
• Uniprot ID: P22314
• Protein Size (# AA): Recombinant
• Molecular Weight: 118 kDa