UAP1 Antibody (OAGA00241)

Catalog No: OAGA00241

Size:100 ul

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
OAGA00241 diluted at 1:3000

Immunohistochemical analysis of paraffin-embedded ES-2 xenograft, using UAP1(OAGA00241) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed A549, using UAP1(OAGA00241) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.
  Positive Controls: H1299
  Cellular Localization: Cytoplasm
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 53 and 473 of UAP1
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: UAP1
• Gene Full Name: UDP-N-acetylhexosamine pyrophosphorylase
• Alias Symbols: AGX, AGX1, AGX2, SPAG2
• NCBI Gene Id: 6675
• Protein Name: UDP-N-acetylhexosamine pyrophosphorylase
• Uniprot ID: Q16222
• Protein Accession #: NP_003106.3
• Molecular Weight: 57 kDa