UACA ELISA Kit (Human) (OKCD09333)

Catalog No: OKCD09333

Size:96WELLS

UACA ELISA Kit (Human) (OKCD09333) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • UACA Microplate: 96 Wells (12 x 8 Well strips)
  • UACA Lyophilized Standard: 2
  • 100X UACA HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.108ng/mL

Target Info

• Gene Symbol: UACA
• Gene Full Name: uveal autoantigen with coiled-coil domains and ankyrin repeats
• Alias Symbols: nuclear membrane binding protein;NUCLING;uveal autoantigen with coiled-coil domains and ankyrin repeats.
• NCBI Gene Id: 55075
• Protein Name: Uveal autoantigen with coiled-coil domains and ankyrin repeats
• Description of Target: Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1 (By similarity).
• Uniprot ID: Q9BZF9
• Protein Accession #: NP_001008225.1
• Nucleotide Accession #: NM_001008224.1