U2AF1 Antibody (OAGA02806)

Catalog No: OAGA02806

Size:100 ul

Sample (30ug whole cell lysate)
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
12% SDS PAGE
OAGA02806 diluted at 1:1000

U2AF35 antibody detects U2AF35 protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
U2AF35 antibody (OAGA02806) diluted at 1:1000.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using U2AF35 (OAGA02806) antibody at 1:200 dilution.

Immunoprecipitation of U2AF35 protein from 293T whole cell extracts using 5 ug of U2AF35 antibody (OAGA02806).
Western blot analysis was performed using U2AF35 antibody (OAGA02806).
EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, IP, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human U2AF35. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.55mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: U2AF1
• Gene Full Name: U2 small nuclear RNA auxiliary factor 1
• Alias Symbols: RN, FP793, U2AF35, U2AFBP, RNU2AF1
• NCBI Gene Id: 7307
• Description of Target: This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Uniprot ID: Q01081