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U2AF1 Antibody (OAAB01511)

400ul
$390.00
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Gene Symbol:
U2AF1
NCBI Gene Id:
7307
Protein Name:
Splicing factor U2AF 35 kDa subunit
Swissprot Id:
Q01081
Protein Accession #:
NP_001020374.1
Alias Symbols:
Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP
Replacement Item:
This antibody may replace item sc-19663 from Santa Cruz Biotechnology.
Description of Target:
U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.
Molecular Weight:
27872 Da (35 kDa subunit) Da
Host:
Rabbit
Clonality:
Polyclonal
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application:
WB, FC, IHC-P
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express U2AF1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express U2AF1.
Immunogen:
This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-125 amino acids from the Central region of human U2AF1.
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Cow; Mouse
Product Format:
Liquid. PBS with 0.09% (W/V) sodium azide.
Reconstitution and Storage:
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals:
Printable datasheet for anti-U2AF1 antibody - OAAB01511
Isotype:
Rabbit Ig
Intended Use:
For research use only and not for use in diagnostic or therapeutic procedures.
Application Info:
WB: 1:1000
IHC-P: 1:50~100
FC: 1:10~50
Additional Information:
Function: Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
Cellular Location: Nucleus. Nucleus speckle.

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1 Item(s)

92/04/2019 06:35
  • Overall Experience:
  • Quality:
HeLa Cell Lysates in WB

Submitted by:
Maja Dembic
Syddansk Universitet

U2AF1

Sample type: HeLa cells

Lane 1: 15ug HeLa nuclear extract

Lane 2: 30ug HeLa nuclear extract

Primary antibody dilution: 1:500 O.N. at 4°C.

Secondary antibody and dilution:

Goat anti-mouse IgG-HRP; 1:10 000.
Goat anti-rabbit IgG-HRP; 1:15 000.

Protocol: Various samples were run and separated on a Bis-Tris 4-12% gradient gel in MOPS 1x buffer, under denaturing conditions. The samples were transferred on PVDF membrane (30V constant;1h, 30 min) and blotted with the antibodies. After blocking for aspecific binding all primary antibodies were incubated O.N., at 4 degrees. Washing and secondary antibody staining was performed on SNAP i.d 2.0 Protein Detection System, Millipore, according to instructions. After incubation with ECL, the membranes were exposed for a maximum of 10 minutes.

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