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Catalog No: OKCA01939
Size:96 Wells
Price: $754.00
SKU
OKCA01939
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
ELISA Kit PrincipleAviva Systems Biology Tyms ELISA Kit (Rat) (OKCA01939) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tyms has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tyms is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Tyms captured in the well.
ELISA Kit Range31.25 - 2,000 pg/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)10499-106
EDTA Plasma (n=4)9388-96
ELISA Kit Component
ComponentAmount
Tyms Microplate96 Wells (12 x 8 Well strips)
Tyms Lyophilized Standard2 x 2 ng
100X Biotinylated Tyms Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity7.81 pg/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolTYMS
Gene Full Namethymidylate synthetase
Alias SymbolsHST422, MGC88736, TMS, TS, TSase|thymidylate synthase
NCBI Gene Id29261
Protein NameThymidylate synthase
Description of TargetContributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Uniprot IDP45352
Protein Accession #NP_062052.1
Nucleotide Accession #NM_019179.1
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