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Catalog No: OKCD02951
Size:96WELLS
Price: $650.00
SKU
OKCD02951
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Datasheets/ManualsPrintable datasheet for TXNRD1 ELISA Kit (Rat) (OKCD02951)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)85-94%89-97%80-103%91-99%
EDTA Plasma (n=5)80-103%98-105%92-99%80-104%
Heparin Plasma (n=5)94-104%93-105%85-96%79-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thioredoxin Reductase 1 (TXNRD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thioredoxin Reductase 1 (TXNRD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thioredoxin Reductase 1 (TXNRD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thioredoxin Reductase 1 (TXNRD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-9484
EDTA Plasma (n=5)84-9789
Heparin Plasma(n=5)78-8981
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thioredoxin Reductase 1 (TXNRD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thioredoxin Reductase 1 (TXNRD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TXNRD1 Microplate96 Wells (12 x 8 Well strips)
TXNRD1 Lyophilized Standard2
100X Biotinylated TXNRD1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.31 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Thioredoxin Reductase 1 (TrxR1). No significant cross-reactivity or interference between Thioredoxin Reductase 1 (TrxR1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolTXNRD1
Gene Full Namethioredoxin reductase 1
Alias SymbolsNADPH-dependent thioredoxin reductase;Thioredoxin reductase TR1.
NCBI Gene Id58819
Protein NameThioredoxin reductase 1, cytoplasmic
Description of TargetThioredoxin reductase 1, cytoplasmic
Uniprot IDO89049
Protein Accession #NP_113802.2
Nucleotide Accession #NM_031614.2
Protein Size (# AA)499
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